Nabeel S. Othman
Two methods have been suggested for the quantitative estimation of Teicoplanin (TCP) as pure and in its dosage forms. The first: spectrophotometric method included reaction of TCP with Fe (III) in acidic medium and the liberated Fe (II) coupled with potassium ferricyanide, the reaction needs heating for 30 minutes at 40o C to form Prussian blue complex. The maximum absorption of resulting product was measured at 767 nm with a molar absorptivity of 6.39 x 105 l.mol-1.cm-1.Linearity according to Beer's law is in the concentration range from 0.4 to 7.5μg . Ml-1. The relative standard deviation% (RSD %) of the method was better than 2.9 % .The LOD and LOQ values have been calculated and equal to 0.1541 and 0.5136 μg.ml-1 respectively. The second method was reverse phase high performance liquid chromatographic (RP-HPLC) using C18 (150 mm length x 4.6 mm ID, 5 μm) column with 20 μl injection volume. The mobile phase was consisting of methanol: Acetonitrile in ratio (90:10 V /V), and the flow rate was maintained at 2.5 ml.min-1.TCP was monitored using Agilent 1200 series equipped with photo diode array detector. Different analytical parameters were determined according to International Conferences on Harmonization (ICH).Linearity was observed in concentration range from 4 to 200. The suggested methods are simple and sensitive and the RPHPLC can be used as a quality-control tool for routine quantitative analysis of TCP in pharmaceutical dosage form.